I think the only way to build a 100 dton ship in the near future is going to be orbital assembly, but suing something like the boosters SpaceX is designing for their Mars colonisation missions, you should be able to build a 100 dton ship in a handful of launches. Their Mars colony ship is supposed to be 100 metric tons.
A big constraint on the design of the vessel depends on what sort of reaction drive you expect the vessle to have. If it's equipped with conventional rockets, then aside from the jump fuel, it's going to need to carry an awful lot of conventional rocket propellant. If it has nuclear propulsion, you can either reduce that quite a bit, or still provide it with a large store of reaction mass and dramatically expand it's dV, enabling a host of mission profiles that would simply not be possible with conventional propulsion.
I think a vehicle like this is likely to be complex enough that you'd need more than 2 crew to operate it and it's subsystems and payloads (mostly probes), probably more like 4 to 6, but you'd be able to get away without staterooms a la conventional Traveller. After all, it's not as if any of our currently planned Mars missions provide for that kind of luxury.
Initial jumps are going to be within the solar system, if only to test and prove the drive system. In fact the first few jumps are likely to be extremely short range, within boost-back range of Earth, in case there's a problem. Once the drive is properly proved, the first interplanetary missions would be tried, either to Mars or Jupiter. These missions would be used to build experience with Jump technology and planetary survey techniques.
A huge problem with the first interstellar jump is knowing exactly where to go. Even assuming we can target a particular destination point very accuractely, we don't know very precisely what planets there are round our neighbouring stars. Furthermore, we don't actualy know very accurately where the stars are. We know the direction to them fairly well, but the distance to them is really just an estimate. I'd be surprised if we know the distances to less than a few hundred AU or so. That means the first jump we make will be a very long range survey mission to obtain astrographic data about the position of the star and it's planets, and perform medium range observations of the planetary system for the second trip. Even on that second trip, you'd not want to risk trying to get too close to any planets, but you would know where they are so you could dispatch a few probes. You'd probably also want to drop off a communications relay in stellar orbit, to collect together all the transmitted data from the probes and store it for remote retrieval whyen you come back.
Only on your 3rd or 4th trip would you risk getting close enough to a planet to attempt boosting the ship into orbit. You'd want to do dozens of trips to the system and very thorough robotic surveys, including surface rovers and perhaps also sample return missions, before considering manned landings. Especially considering that manned landings on anything much bigger than Mars, or with a substantial atmosphere, would be one-way trips.
Simon Hibbs